Why use Ropods
-
Plants are grown and imaged within the same chamber, thus there is no mechanical stress during sample mounting and imaging
-
RoPods prevents samples from drying up during imaging allowing for long-term time-lapse series
-
Plant growth within RoPod requires very small volume of the medium, thus drug treatment will require a small amount of compounds
-
The watertight RoPods allow drug treatment in chambers positioned vertically and horizontally. So you can shuttle your RoPod chambers from growth conditions to a microscope and back while the treatment is ongoing without stressing the plants.
-
RoPods allows multiple biological replicates being imaged simultaniously (up to 18 seedlings in a RoPod 5)
-
Plants are immobilized within the chamber, thus the same plant can be easily tracked in a RoPod in multiple imaging sessions
-
RoPods 4 and 5 have separators for each root to prevent crossing during growth and to make easier tracking biological replicates
-
RoPods cost almost nothing, they are printed using PETG plastic and 24x60mm microscopy coverglass (#1.5, i.e. 0.16-0.19 mm thick)
-
The glass is printed into the plastic and unlike commercially available chambers RoPods would never unglue apart in the middle of your experiment